Alpha-thrombin is a product of calf plasma prothrombin activated by FXa (assisted by FVa and phospholipids), purified by ion exchange and gel filtration chromatography, and inactivated by S/D virus.

Thrombin, also known as Factor IIa (FIIa), plays a very important role in the coagulation waterfall as a serine protease.

The Alpha-thrombin reagent produced by Beijing Asler Biotechnology Co., Ltd. is specially optimized for the detection of heparin anti-FIIa titer, and the main configuration is Alpha-thrombin, which is very beneficial to the detection method due to its high purity and high stability.

Form and composition

Alpha-thrombin is a lyophilized product.

Key ingredients

Alpha-thrombin 50 IU

Filler

TRIS 60.5 mg

Physical and chemical properties

Appearance

White freeze-dried powder

Molecular weight

approx. 36 kDa

Percent absorbance coefficient (l=1 cm, λ=280 nm, 1%)

about 10

Isoelectric point

7.0

Specific activity

≥700 IU/mg protein

How to use

Add an appropriate amount of water or buffer to obtain the desired concentration of Alpha-thrombin solution according to application requirements.

Stability and storage methods

Alpha-thrombin lyophilized products should be stored at 2-8°C in a dark place and used within the expiration date indicated on the label;

5 IU/mL Alpha-thrombin solution is stored at 2-8°C in a dark place for about 7 days.

Notes

Alpha-thrombin is suitable for the detection of anti-FIIa activity of anticoagulant drugs such as heparin, and is only used for experimental research and industrial quality control, and cannot be used as a drug or in vivo experiment.

Ordering Information

Alpha-thrombin is sold by the bottle.

Order number

AS00-0122

Package size

50 IU

Delivery time

In stock

Application Examples

Alpha-thrombin has a wide range of uses, such as

• The ratio of anti-FIIa activity and anti-FXa activity of unfractionated heparin and low molecular weight heparin was detected
• Accurate detection of anti-FIIa activity of unfractionated heparin and low molecular weight heparin
• Monitor the amount of heparin in blood samples
• Tests the level of antithrombin in the blood to diagnose antithrombin deficiency

As an affinity column stationary phase, proteins with thrombin affinity are isolated and purified

About the color substrate method

Alpha-thrombin (FXa) can be used to detect the anti-FIIa activity of heparin (chromogenic substrate method).

Chromogenic substrates are small chemically synthesized peptides with chromogenic groups (pNA) at one end. Specific enzymes (such as thrombin) catalyze to dissociate pNA. Free pNA can be detected by a spectrophotometer or microplate reader to determine the activity of the corresponding substance.

Common chromogenic substrates are: thrombin chromogenic substrate S-2238, FXa chromogenic substrate S-2765 and S-2222.

Chromogenic assays are currently the main methods for detecting heparin titers, which are the methods for the detection of heparin (especially low molecular weight heparin) specified in the European Pharmacopoeia (EP) and the United States Pharmacopoeia (USP). This method is also widely used in laboratory research and in vitro diagnostics.

By controlling the conditions of the reaction system, chromogenic substrate methods can detect clotting factors, cofactors, or clotting factor inhibitors.