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Название компании :
Пекинская компания по биотехнологиям Asnail, ОООBrand :
Asnail
Категории продуктовАнализ активности гепарина
①Heparinases
Heparinases can clave glycosidic bonds of heparin and/or heparan sulfate by a β-elimination mechanism, generating unsaturated products (mostly
disaccharides) with a double bond be-tween C4 and C5 of the uronate residue.
Features:
● Natural heparinase, from Flavobacterium heparinum.
● It has the highest specific activity compare with other products on the market.
● Pure enzyme, free of BSA or other protein impurities.
● Good stability, consistent batch reproducibility and degradation characteristics.
②TOSOH TSKgel SWXL Columns
Tosoh SWxl series columns feature high porous volume/unit volume ratio, low sample absorption, and high column efficiency. Could be used for MW
determinations of heparin and LMWHs.
③BioCore™ SEC columns
Asnail co-developed the BioCore SEC-HP columns, tailored for molecular weight (MW) and MW distribution. The column is durable and can provide
better volume exclusion separation.
④Dionex™ IonPac™ AS11 IC Columns
The Column specifically designed to resolve a large number of inorganic anions and organicacid anions from a single sample injection in one gradient run using hydroxide eluent systems.USP officially specifies the method for the identification of heparin sodium chromatography.
Alpha-thrombin is a product of calf plasma prothrombin activated by FXa (assisted by FVa and phospholipids), purified by ion exchange and gel filtration chromatography, and inactivated by S/D virus.
Thrombin, also known as Factor IIa (FIIa), plays a very important role in the coagulation waterfall as a serine protease.
The Alpha-thrombin reagent produced by Beijing Asler Biotechnology Co., Ltd. is specially optimized for the detection of heparin anti-FIIa titer, and the main configuration is Alpha-thrombin, which is very beneficial to the detection method due to its high purity and high stability.
Form and composition
Alpha-thrombin is a lyophilized product.
Key ingredients
Alpha-thrombin 50 IU
Filler
TRIS 60.5 mg
Physical and chemical properties
Appearance
White freeze-dried powder
Molecular weight
approx. 36 kDa
Percent absorbance coefficient (l=1 cm, λ=280 nm, 1%)
about 10
Isoelectric point
7.0
Specific activity
≥700 IU/mg protein
How to use
Add an appropriate amount of water or buffer to obtain the desired concentration of Alpha-thrombin solution according to application requirements.
Stability and storage methods
Alpha-thrombin lyophilized products should be stored at 2-8°C in a dark place and used within the expiration date indicated on the label;
5 IU/mL Alpha-thrombin solution is stored at 2-8°C in a dark place for about 7 days.
Notes
Alpha-thrombin is suitable for the detection of anti-FIIa activity of anticoagulant drugs such as heparin, and is only used for experimental research and industrial quality control, and cannot be used as a drug or in vivo experiment.
Ordering Information
Alpha-thrombin is sold by the bottle.
Order number
AS00-0122
Package size
50 IU
Delivery time
In stock
Application Examples
Alpha-thrombin has a wide range of uses, such as
As an affinity column stationary phase, proteins with thrombin affinity are isolated and purified
About the color substrate method
Alpha-thrombin (FXa) can be used to detect the anti-FIIa activity of heparin (chromogenic substrate method).
Chromogenic substrates are small chemically synthesized peptides with chromogenic groups (pNA) at one end. Specific enzymes (such as thrombin) catalyze to dissociate pNA. Free pNA can be detected by a spectrophotometer or microplate reader to determine the activity of the corresponding substance.
Common chromogenic substrates are: thrombin chromogenic substrate S-2238, FXa chromogenic substrate S-2765 and S-2222.
Chromogenic assays are currently the main methods for detecting heparin titers, which are the methods for the detection of heparin (especially low molecular weight heparin) specified in the European Pharmacopoeia (EP) and the United States Pharmacopoeia (USP). This method is also widely used in laboratory research and in vitro diagnostics.
By controlling the conditions of the reaction system, chromogenic substrate methods can detect clotting factors, cofactors, or clotting factor inhibitors.
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